JNDA Vol. 22 No. 1 Issue 34 (January - June 2022) |
Tissue Eosinophilia as Histopathological Marker in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma |
Dr. Dipshikha Bajracharya, Dr. Bidhata Ojha, Dr. Pradeep Bhandari, Dr. Akanksha Badhu, Dr. Dhana Kumari Manadhar |
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Abstract |
Introduction: Oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are common lesions. Correlation of tissue eosinophilia in OPMDs and OSCCs has shown varied results as a diagnostic and prognostic indicator. Objective: To evaluate tissue eosinophilia in oral potentially malignant disorders and oral squamous cell carcinoma and its possibility as histopathological marker using Congo red stain. Materials and Method: Analytical cross-sectional study in 100 histologically diagnosed cases of OPMDs, OSCCs and normal mucosa was done at Kantipur Dental College and Teaching hospital from Jan 2022 to May 2022. Convenience sampling technique was utilised. Cases and controls were stained with haematoxylin and eosin (H&E) and Congo red. Eosinophils were counted and recorded as eosinophils/10 hpf. Mean eosinophil count and mean difference between normal mucosa, OPMDs and OSCCs were compared and analysed using SPSS v.21. Result: Mean difference in eosinophil count between different grades of dysplasia was statistically significant. There was statistically significant mean difference in eosinophil count between OSCC, dysplasia, OSMF, lichen planus, and normal mucosa. The mean eosinophil count was compared between H&E and Congo red stain in cases and control which showed significant difference. Conclusion: Increased tissue eosinophilia from normal to OPMDs to OSCCs was found suggesting it might have role in stromal invasion. |
Keywords |
Eosinophils; oral potentially malignant disorders; oral squamous cell carcinoma. |