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Objective: An in vitro evaluation of the pH and calcium ion release exhibited by different calcium hydroxide based sealers, Sealapex, Acroseal and Endoflas F.S. from simulated root canal apical openings.
Study Design: The sealers were mixed according to the manufacturer’s instructions and placed in the eppendorf tubes of 0.3 and 0.5 mm diameter aperture. Tubes were then immersed in flasks containing double distilled water. The solution was tested for pH by pH meter and calcium ion release by spectrophotometer at 24hrs, 48hrs, 5th day, 7th day, 15th day and 28th day intervals. The data obtained was statistically analysed using ANOVA.
Results: Seal apex showed highest pH and calcium ion release followed by Acroseal and then Endoflas F.S.in both 0.3 and 0.5 diameter aperture tubes.
Conclusion: Sealapex showed highest pH and calcium ion release followed by Acroseal and then Endoflas F.S.

acroseal , atomic absorption spectrophotometer, calcium hydroxide, sealapex, endoflas f.s.